Fig. 4

Functional analysis of iSGC. a Representative immunofluorescence of CK18⁺ and AQP5⁺ in HDF and iSGC. Scale bar = 50 μm. b Percentages of CK18⁺ and AQP5⁺ cells in HDF and iSGC calculated according to the immunostaining. Quantification was done with 5 randomly selected microscopy fields from each of the 3 independent experiments. c Calcium activity analysis was used to assess the reactivity to acetylcholine. d The data presented the intracellular free Ca²⁺ intensity of iSGCs was higher than HF-EDA in SGM and similar to that of the pSGC, (60.79 ± 7.71)%, (12.65 ± 2.07)% and (70.59 ± 0.34)%, respectively. n = 3. Data were expressed as mean ± SD and analyzed by two-tailed t-tests, ^**P < 0.01, ^***P < 0.001, ns not significant, HDF human dermal fibroblasts, iSGC induced sweat gland-like cell, CK18 cytokeratin 18, AQP5 aquaporin 5, EDA ectodermal dysplasia antigen, SGM sweat gland culture medium, pSGC primary sweat gland cell, ns not significant