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Fig. 1 | Military Medical Research

Fig. 1

From: Ultrasound-triggered microbubble destruction enhances the radiosensitivity of glioblastoma by inhibiting PGRMC1-mediated autophagy in vitro and in vivo

Fig. 1

UTMD enhanced the radiosensitivity of glioblastoma cells. a GL261 and U251 cells were exposed to different doses of IR (2, 4, 8, and 10 Gy), and after 24 h, cell viability was measured using a CCK-8 detection kit. b Cells were treated at ultrasonic intensities of 0.4, 0.8, 1.2 and 2.0 W/cm2 at a duty cycle of 10% for 60 s and then incubated for another 24 h, and the cell viability of different experimental groups was detected using the CCK-8 detection kit. c Cells were treated with MBs (50, 100, 200, and 400 μl/ml) at an intensity of 1.2 W/cm2 and a duty cycle of 10% for 60 s. Twenty-four hours later, cell viability was measured using a CCK-8 detection kit. d, e GL261 and U251 cells were treated at ultrasonic intensities of 0.4, 0.8, 1.2 and 2.0 W/cm2 with different concentrations of MBs (50, 100, 200, and 400 μl/ml) at a duty cycle of 10% for 60 s. Then, the cells were exposed to IR (2 Gy). After 24 h, the cell viability of different experimental groups was detected using the CCK-8 detection kit. f, g GL261 and U251 cells were treated with UTMD-L (ultrasonic intensity of 1.2 W/cm2 combined with 100 μl/ml MBs) or UTMD-H (ultrasonic intensity of 1.2 W/cm2 combined with 200 μl/ml MBs) at a duty cycle of 10% for 60 s, and then the cells were exposed to IR (2 Gy). After 24 h, cell death was determined by PI staining followed by flow cytometry. h The quantifications of cell death. Values are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01 vs. the vehicle-treated control group; #P < 0.05, ##P < 0.01 vs. IR group. UTMD ultrasound-triggered microbubble destruction, IR ionizing radiation, MBs microbubbles, CCK-8 cell counting kit-8, PI propidium iodide, SD standard deviation

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