Fig. 3
Schematic diagram for multiplex detection of CRP, PCT, and IL-6 using WMC-MDP. a Prior to detection, pretreatment includes coating capture antibody strips, surface preparation of channels, and the addition of reagents. b Premixing samples with detection antibodies in wave-shaped micromixer. c Sandwich immunoassay occurs in the detected channels and forms structures of Ab1-CRP-Ab2-HRP, Ab1-PCT-Ab2-HRP, and Ab1-IL-6-Ab2-B-SA-HRP. d Removing unreacted reagents by washing channels. e Adding CL substrate into the channels to produce CL intensity. CRP c-reactive protein, PCT procalcitonin, IL-6 interleukin-6, WMC-MDP wave-shaped microfluidic chip assisted multiplexed detection platform, PBST phosphate buffered saline tween-20, CL chemiluminescence, BSA bovine serum albumin, CRP-Ab1 capture antibodies of c-reactive protein, PCT-Ab1 capture antibodies of procalcitonin, IL-6-Ab1 capture antibodies of interleukin-6, CRP-Ab2 detection antibodies of c-reactive protein, PCT-Ab2 detection antibodies of procalcitonin, B-IL-6-Ab2 detection antibodies of interleukin-6 conjugated with biotin, HRP horseradish peroxidase, SA-HRP horseradish peroxidase conjugated with streptavidin