Fig. 5

rm-FGF2 intranasal instillation promoted HDM-induced airway inflammatory cell infiltration and recruited subepithelial neutrophils in the mouse model. a Representative H&E staining images for the lung sections. Scale bar for the bronchial airway images = 100 μm and for the alveolar area images = 50 μm. b Score of airway inflammatory cell infiltration levels between the HDM and HDM + rm-FGF2 groups (n = 7). c ELISA results showing the total IgE and anti-HDM-specific IgE levels in the sera among experimental groups (n = 4 or 7). d Analyses of the transcription levels for IL-4, IL-10, and IL-13 genes in each group through RT-qPCR. mRNA levels were normalised to the housekeeping gene, Rpl13a. e Representative immunofluorescence images and statistical analysis showing that the number of subepithelial neutrophils was increased in the HDM + rm-FGF2 group compared with that in HDM alone group (n = 5, scale bar = 20 μm). Data are represented as mean ± standard error of the mean. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001. AW airway, rm-FGF2 recombinant mouse FGF2 protein, HDM house dust mite