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Fig. 7 | Military Medical Research

Fig. 7

From: Excellent effects and possible mechanisms of action of a new antibody–drug conjugate against EGFR-positive triple-negative breast cancer

Fig. 7

Effects of LR004-VC-MMAE on EGFR, EGFR downstream signal and the expression of tumor stemness marker genes. a Stability of EGFR protein in MDA-MB-231 cells. The cells pretreated with 2.5 nmol/L LR004-VC-MMAE or PBS (as a control) were incubated with cycloheximide (CHX) (20 mmol/L) for indicated durations. The EGFR protein levels were evaluated by immunoblotting. Representative images (left panel) and protein degradation curves (right panel) are shown. b Analysis of EGFR downstream signaling. Serum-starved MDA-MB-231 cells were treated with 2.5 nmol/L LR004-VC-MMAE or PBS for 24 h, followed by EGF (100 ng/ml) stimulation for 30 min. The indicated proteins and their phosphorylation status were evaluated by immunoblotting. c Expression of stem cell maker genes. MDA-MB-231 cells were treated with PBS or LR004-VC-MMAE (2.5 or 5 nmol/L) for 24 h. The indicated proteins were detected by immunoblotting

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