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Fig. 7 | Military Medical Research

Fig. 7

From: Propofol postconditioning ameliorates hypoxia/reoxygenation induced H9c2 cell apoptosis and autophagy via upregulating forkhead transcription factors under hyperglycemia

Fig. 7

Knockout of propofol FoxO1 or FoxO3a canceled the protective effects of propofol in attenuating the augmentation of ROS Levels caused by H/R injury under hyperglycemia (35 mmol/L). a ROS (reactive oxygen species) degrees measured by DHE (dihydroethidium) fluorescence staining (100 ×). b ROS levels detected by DCFH-DA (2, 7-dichlorodihydrofluorescein diacetate) fluorescence flow cytometry. c Statistical graph of DHE. d Statistical image of mean DCFH-DA fluorescence intensity. Data are mean ± SEM from three independent experiments each performed in triplicate. *P < 0.05, **P < 0.01

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