Fig. 1
From: Advances in single-cell sequencing: insights from organ transplantation
Steps of scRNA-seq to analyzing organ rejection. After biopsies of graft tissues, cells are isolated and can be used for droplet- or plate-based sequencing approaches. After batch effect correction, normalization, data imputation and dimensionality adjustments, specific cellular subtypes can be identified. Analysis of heterogeneity and prediction of tolerance is used to identify target genes and molecular interactions that are the basis for gene therapy approaches and long-term graft acceptance. QC quality control; DCs dendritic cells