Table 1 Primer sequences for qPCR
From: RIG-I, a novel DAMPs sensor for myoglobin, activates NF-κB/caspase-3 signaling in CS-AKI model
Gene names | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
Rat KIM-1 | GGTCTGTATTGTTGCCGAGTGGAG | GCCTTGTGGTTGTGGGTCTTGTAG |
Rat NGAL | CAGGGCAGGTGGTTCGTTGTC | CGAGGATGGAAGTGACGTTGTAGC |
Rat RIG-I | GGCTGACTGCTTCCGTTGGTG | CGAGGGCGGCACAGAGTTTG |
Rat IRF3 | CTTACGACAGGACGCACAGATGG | CAGGTTGACAGGTCTGGCTTATCC |
Rat P65 | AACACTGCCGAGCTCAAGAT | CATCGGCTTGAGAAAAGGAG |
Rat IFN-β | TTGCGTTCCTGCTGTGCTTCTC | TCCGTCCTGTAGCTGAGGTTGAG |
Rat TNF-α | AAATGGGCTCCCTCTCATCAGTTC | TCTGCTTGGTGGTTTGCTACGAC |
Rat IL-6 | ACTTCCAGCCAGTTGCCTTCTTG | TGGTCTGTTGTGGGTGGTATCCTC |
Rat caspase-3 | AACGGACCTGTGGACCTGAA | TCAATACCGCAGTCCAGCTCT |
Rat β-actin | TCACCCACACTGTGCCCATCTATGA | CATCGGAACCGCTCATTGCCGATAG |