Fig. 4
From: RIG-I, a novel DAMPs sensor for myoglobin, activates NF-κB/caspase-3 signaling in CS-AKI model
Knocking down RIG-I gene could alleviate the myoglobin induced NF-κB/caspase-3 axis activation. a. Co-IP showed the interaction between RIG-I and myoglobin in the rat kidney of the CS group. b–d. qPCR, and Western blotting analyses RIG-I expression after NRK-52E cells treatment with siRIG-I. Fig. d is the quantification of Fig. c. e–i. qPCR, and Western blotting analyses cells RIG-I, IRF3, p-IRF3, P65, p-P65, caspase-3, and cleaved caspase-3 expression after treatment with ferrous myoglobin and siRIG-I. Fig. i is the quantification of Fig. h. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired t-test or by one-way ANOVA followed by the Brown–Forsythe multiple comparisons test, respectively