Fig. 6

Verification of the hsa_circ_0065336/miR-505-3p/PTPN11 regulatory pathway. a-c. The mRNA expression levels of hsa_circ_0065336 (a), miR-505-3p (b) and PTPN11 (c) were determined by RT-qPCR at the indicated time points. d. Putative binding sites of hsa_circ_0065336 and miR-505-3p. e. Dual-luciferase reporter assay between hsa_circ_0065336 and miR-505-3p. f. Putative binding sites of miR-505-3p and PTPN11. g. Dual-luciferase reporter assay between miR-505-3p and PTPN11. h. The PTPN11 expression level was regulated by hsa_circ_0065336 and miR-505-3p. ^*P < 0.05; ^**P < 0.01. MT: Wild type; MUT: Mutant type; NC: Negative controls