Fig. 2
DIRAS3 and p53 re-expression modulates downstream genes involved in apoptosis, proliferation and cell cycle arrest. a. CAL-27 cells were treated with Ad-DIRAS3, rAd-p53, and their combination. Ad-GFP was used as a negative control. After 24 h of infection, whole cell lysates were collected, and the expression of DIRAS3 and p53 was detected by western blotting. b. Relative mRNA expression levels of DIRAS3 and p53 were determined by real-time PCR and calculated by normalizing to GAPDH. The fold changes are relative to the control group, to which a value of 1.0 was assigned. c. The expression of the three genes p21WAF1/CIP1, Bax and STAT3 was examined by Western blotting after adenovirus infection. d. Relative mRNA levels of p21WAF1/CIP1, Bax and STAT3 were detected by real-time PCR. ***P < 0.001