Fig. 6

The electrophysiological properties and phenotypic characteristics of ALS-As-iMNs. a A current clamp recording showed repetitive firing of action potentials in response to the injection of an 800 ms current pulse (40 pA) in wild type-MNs (WT-MNs) isolated from healthy mouse spinal cords and ALS-As-iMNs (WT-MNs, n = 8/12 recorded cells; ALS-As-iMNs, n = 6/10 recorded cells). b Representative traces of whole-cell current in the voltage-clamp mode in response to depolarizing voltage steps from a holding potential of − 60 at 10-mV increments, showing inward sodium current and outward potassium current (WT-MNs, n = 9/12 recorded cells; ALS-As-iMNs, n = 8/10 recorded cells). c The phenotypes of ALS-As-iMNs and WT-MNs after culturing for 4 weeks. d The cell survival rate of ALS-As-iMNs and WT-MNs cultured for 3 and 4 weeks compared to that observed for ALS-As-iMNs cultured for 2 weeks (means ± SEM from three independent experiments, *P < 0.05). e and f Quantification of the cells producing ROS among ALS astrocytes (ALS-As), ALS-As-iMNs, wild-type astrocytes (WT-As) and WT-MNs isolated from healthy mouse spinal cords (means ± SEM from triplicate samples, **P < 0.01). g Colorimetric measurement of LDH (normalized to gDNA) in the supernatants of ALS-As-iMN and WT-MN cultures (means ± SEM from triplicate samples, *P < 0.05)