Fig. 1

The flow chart of all experiments. Eight mice of M. vaccae group or control group were challenged with M. tuberculosis H37Rv strain. After 3 times immunization, mice were sacrificed and their lungs, livers, and spleens were collected to efficacy evaluation and pathological observation. The PBMCs of 3 mice of each group were isolated to extract total RNA. Hierarchical Clustering was performed to show the distinguishable gene expression profiling between samples. DE genes with statistical significance were identified through Volcano Plot filtering. Finally, pathway analysis and GO Analysis were applied to determine the roles of these DE genes played in these biological pathways or GO terms. GO, Gene Ontology; PBMCs, peripheral blood mononuclear cells; CFUs, colony formation units