Results of Western blotting. This figure shows the changes in the protein expression of HIF-1α, TGF-β and VEGF-A in wound tissue; β-actin was used as a loading control. a-d. The relative protein expression of HIF-1α, TGF-β1 and VEGF-A was calculated as integrated density values. The error bars indicate the standard deviation of the mean. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with control group.