Fig. 5

IL-25 suppresses exosome release from AMϕ in vivo. a and b WT mice were given intratracheal (i.t.) LPS (2 mg/kg BW in a volume of 100 μl/mouse) or sham (i.t. PBS, 100 μl/mouse) for 24 h, and BALF was collected. IL-25 was measured in the supernatant by ELISA (a) and the surface expression of IL-25R on AMϕ was detected by flow cytometry (b). c WT mice were treated with LPS (2 mg/kg BW in a volume of 100 μl/mouse, i.t.), sham (PBS, 100 μl/mouse, i.t.), LPS (2 mg/kg BW, i.t.) + IgG (1 mg/kg BW, i.t.); or LPS (2 mg/kg BW, i.t.) + anti-IL-25 antibody (1 mg/kg BW, i.t.) for 24 h. Exosomes were isolated from BALF and quantified by CD63 staining and flow cytometry. All results are representative of three independent experiments. The graphs show the mean ± SEM, n = 3; *P < 0.05 or **P < 0.01, compared with the indicated groups or with the control