Solution | pH buffer | pH gelatin | pH buffer/gelatin | Buffer composition |
---|---|---|---|---|
RO water | 4.6 | 4.8 | N/A | N/A |
PBS | 7.0 | 4.8 | 6.5 | 137 mmol/L NaCl, 2.7 mmol/L KCl, 10 mmol/L Na2HPO4, 1,8 mmol/L KH2PO4 |
M9 | 7.0 | 6.5 | 7.0 | 22 mmol/L KH2PO4, 42 mmol/L Na2HPO4, 86 mmol/L NaCl |
M63 | 7.0 | 6.5 | N/A | 100 mmol/L KH2PO4, 15 mmol/L (NH4)2SO4, 1 mmol/L MgSO4, 1.7 μmol/L FeSO4 |
Sodium phosphate pH 8 | 8.0 | 7.3 | 7.8 | 93 mmol/L Na2HPO4, 7 mmol/L NaH2PO4, |