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Fig. 5 | Military Medical Research

Fig. 5

From: PM2.5 obtained from urban areas in Beijing induces apoptosis by activating nuclear factor-kappa B

Fig. 5

Effects of PM2.5 and an NF-κB activator/inhibitor on BAD protein expression. a. Cells were treated with 100, 200, and 400 μg/ml PM2.5. b. Cells were treated with 200 μg/ml PM2.5 and an NF-κB activator (100 ng/ml LPS) or inhibitor (100 μmol/L PDTC). CHO cells were cultured in 6-well plates at a density of 5.0 × 104 cells/well in 2 ml of F12-K medium supplemented with 10% FBS and incubated overnight. The media was changed, and the cells were treated with test samples for 24 h. Western blotting was performed. Each blot of BAD protein was reprobed for β-actin as a loading control. Values are expressed as the mean ± SD. Each data point represents an average of three samples. Compared with the untreated control, * P < 0.05; compared with PM2.5 200 μg/ml, # P < 0.05; compared with LPS, Δ P < 0.05

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