Table 1 Primers and probes used for pathogen detection in acute respiratory infections
Pathogen | Name of primer | Sequence (5′-3′) |
|---|---|---|
Influenza A virus | QFluA-F | GAC CRA TCC TGT CAC CTC TGA C |
QFluA-R | GGG CAT TYT GGA CAA AKC GTC TAC G | |
QFLuA-Probe | FAM- TGC AGT CCT CGC TCA CTG GGC ACG –BHQ1 | |
Influenza B virus | QFluB-F | TCC TCA ACT CAC TCT TCG AGC G |
QFluB-R | CGG TGC TCT TGA CCA AAT TGG | |
QFLuB-Probe | FAM- CCA ATT CGA GCA GCT GAA ACT GCG GTG -BHQ1 | |
Influenza A seasonal H1 | QH1F | AAC ATG TTA CCC AGG GCA TTT CGC |
QH1R | GTG GTT GGG CCA TGA GCT TTC TTT | |
QH1-Probe | FAM-GAG GAA CTG AGG GAG CAA TTG AGT TCA G-BHQ1 | |
Influenza A seasonal H3 | QH3F | ACC CTC AGT GTG ATG GCT TCC AAA |
QH3R | TAA GGG AGG CAT AAT CCG GCA CAT | |
QH3-Probe | FAM-ACG CAG CAA AGC CTA CAG CAA CTG T-BHQ1 | |
Human adenovirus group B | qHAdV-UniF | TTTGAGGTYGAYCCCATGGA |
qHAdV-UniR | AGAASGGTGTRCGCAGGTA | |
qHAdV-UniProbe | FAM-ACCACGTCGAARACTTCGAA-BHQ1 | |
Human adenovirus type 3 | qHAdV3-F | GGGAGACAATATTACTAAAGAAGGTTTGC |
qHAdV3-R | CAACTTGAGGCTCTGGCTGATA | |
qHAdV3-Probe | FAM-CACTAC“T*”GAAGGAGAAGAAAAGCCCATTTATGCC | |
Human adenovirus type 4 | qHAdV4-F | AAGCCAACCTGTGGAGKAACT |
qHAdV4-R | TGTTGGCCGGTGTGTATTTG | |
qHAdV4-Probe | FAM-CTSTATGCCAATGTTGCCCTCTATTTGCCT-BHQ1 | |
Human adenovirus type 7 | qHAdV7-F | GAGGAGCCAGATATTGATATGGAATT |
qHAdV7-R | AATTGACATTTTCCGTGTAAAGCA | |
qHAdV7-Probe | FAM-AAGCTGCTGACGCTTTTTCGCCTGA-BHQ1 | |
Human adenovirus type 11/55 | qHAdV55-F | CGGAGCAGCCAAATCAGAA |
qHAdV55-R | CATGAGTGTCTGGAGTTTCCAAAT | |
qHAdV55-Probe | FAM-TGCGGCATCACAGAAAACAAACTTAAGTC-BHQ1 | |
Human adenovirus type 14 | qHAdV14-F | GAAAATCATGGTGTGGAAGATGAA |
qHAdV14-R | CAAGCTTGGTCTCCATTTAACTGA | |
qHAdV14-Probe | FAM-ACGGCATCGGTCCGCGAACA-BHQ1 | |
Internal reference | QRnaseP-F | AGA TTT GGA CCT GCG AGC G |
QRnaseP-R | GAGCGGCTGTCTCCACAAGT | |
QRnaseP-P | FAM-TTCTGACCTGAAGGCTCTGCGCG-BHQ1 |