Figure 4

Dynamic changes in the plasma levels of TNF-α, IL-1β, IL-6 and IL-10 in mice exposed to sulfur mustard (SM). Male KM mice were exposed to saline, propylene glycol (PG) and 20 mg/kg SM by subcutaneous injection. Six animals from each group were sacrificed after SM exposure at 4 h, 24 h, 72 h and 168 h, blood samples were collected and plasma samples were obtained as described in Materials and Methods. The IL-1β (A), IL-6 (B), IL-10 (C) and TNF-α (D) levels in plasma were assayed and analyzed by Luminex200. Data are presented as the mean ± SD, n = 6 for each group (A-D). *Significant difference between the PG treatment and SM exposure groups (*P < 0.05, **P < 0.01, ***P < 0.001).