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Fig. 1 | Military Medical Research

Fig. 1

From: CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations

Fig. 1

Construction of the accurate and sensitive mutation analysis platform. a The working mechanism of the method for identifying single base mutation. b SYBR Green I signal during chain extension process. c Fluorescence spectrum of the “Reporter” in the presence or absence of target sequence. d Fluorescence spectrum of the approach when detecting different concentrations of synthesized KRAS-G12D fragment. e Correlation between the recorded fluorescence intensities and the concentration of synthesized KRAS-G12D fragment. f Signal recovery rate of the approach for different mutated sequences detection. g Correlation between the calculated concentration by the method and by the polymerase chain reaction (PCR). *P < 0.05. CRISPR-Cas9 clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9, crRNA CRISPR RNA, PAM protospacer adjacent motif

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Military Medical Research

ISSN: 2054-9369