Fig. 8

EndoA2-siRNA impairs tactile sense and mechanical hypersensitivity in nonhuman primates. a Monkeys were administered EndoA2-siRNA via intrathecal injection (i.t.), and then the behaviors were tested at different time points. Touch sensitivity changes in monkeys after i.t. of EndoA2-siRNA (5, 10, and 20 nmol) in the cotton (b) and foot tape (c) tests. n = 4. The effects of EndoA2-siRNA (20 nmol) on the punctate mechanical hypersensitivity (d) and dynamic mechanical hypersensitivity (e) induced by CFA in monkeys. n = 4. f Colocalization of KIF5B, EndoA2 and Piezo2 in human thoracic DRG sections. Scale bar = 200 μm. g High-resolution images showing the colocalization of KIF5B, EndoA2 and Piezo2 in human thoracic DRG neurons. Arrows indicate colocalization. Scale bar = 10 μm. Two-way ANOVA followed by Bonferroni’s multiple comparisons test (b–e). ^**P < 0.01, ^***P < 0.001. The error bars indicate the SEMs. EndoA2 endophilin A2, NT nontargeting, CFA complete Freund’s adjuvant, DRG dorsal root ganglion