Fig. 6

EndoA2 controls Piezo2 trafficking and mechanical sensitivity via the SH3 domain. a–d The effects of overexpressing the SH3 domain in NF200-positive (NF200⁺) DRG neurons on the expression of Piezo2 in the DRG membrane fraction, cytoplasmic fraction and total lysate. n = 5. e The effects of overexpressing the SH3 domain in NF200⁺ DRG neurons on the trafficking ratio of Piezo2 in DRGs. n = 5. The influence of overexpressing the SH3 domain in NF200⁺ DRG neurons on mechanical sensitivity was evaluated by various von Frey filament (f), cotton (g) and foot tape (h) tests. n = 7. i–l The effects of overexpressing the SH3 domain in NF200⁺ DRG neurons on the punctate and dynamic mechanical allodynia induced by SNL and CFA. n = 7. m, n Overexpression of the SH3 domain in NF200⁺ DRG neurons did not alter the touch sensitivity of EndoA2^fl/fl × Nefh-Cre mice. n = 9. o, p The effects of overexpressing the SH3 domain in NF200⁺ DRG neurons on CFA-induced mechanical allodynia in EndoA2^fl/fl × Nefh-Cre mice. n = 7. Two-tailed independent Student’s t test (d, e, g, h, m, n); Two-way ANOVA followed by Bonferroni’s multiple comparisons test (f, i–l, o, p). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns non-significant. The error bars indicate the SEMs. TfR transferrin receptor, SNL spinal nerve ligation, CFA complete Freund’s adjuvant, EndoA2 endophilin A2