Fig. 3

EndoA2 in NF200-positive (NF200⁺) medium-to-large diameter sensory neurons is necessary for tactus and mechanical allodynia. Conditional knockout and re-expression of EndoA2 in NF200⁺ DRG neurons impaired and rescued SNL-induced punctate mechanical hypersensitivity (a) and dynamic mechanical hypersensitivity (b), respectively. n = 6. c–f Conditional knockout and re-expression of EndoA2 in NF200⁺ DRG neurons impaired and rescued CFA- and VCR-induced punctate mechanical allodynia and dynamic mechanical allodynia, respectively. n = 6. g The percentage of withdrawal for von Frey filaments under a range of forces in EndoA2^fl/fl, EndoA2^fl/fl × Nefh-Cre and rescue mice. n = 6. ^†P < 0.05, ^††P < 0.01, ^†††P < 0.001 indicates EndoA2^fl/fl vs. EndoA2^fl/fl × Nefh-Cre; ^#P < 0.05, ^###P < 0.001 indicates EndoA2^fl/fl × Nefh-Cre vs. rescue. The behaviors of EndoA2^fl/fl, EndoA2^fl/fl × Nefh-Cre and rescue mice were evaluated by cotton (h) and foot tape (i) tests. n = 6. One-way ANOVA followed by Tukey’s multiple comparisons test (h, i); Two-way ANOVA followed by Bonferroni’s multiple comparisons test (a–g). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns non-significant. The error bars indicate the SEMs. EndoA2 endophilin A2, SNL spinal nerve ligation, CFA complete Freund’s adjuvant, VCR vincristine