Fig. 3

PEGDA–PLL matrix efficiently immobilizes lentiviruses (LVs) and delivers genes in vivo. a Percentage of GFP expression in LentiX cells when incubated for 72 h with LVs released at 3 h and 6 h from matrices immobilized with different numbers of lentiviral particles (10⁶, 10⁷, and 10⁸). b Schematic of Luc-RFP plasmid construct used to produce luciferase encoding LVs. c Experimental design of bioluminescence study conducted to visualize temporal expression of luciferase gene by delivering Luc-LVs either in a bolus manner (subcutaneous injection) or via immobilization on PEGDA–PLL implant. d Representative in vivo bioluminescence imaging of 3 mice per group shown after injecting D-luciferin 150 mg/kg body weight of mice. e Average radiance measured using constant-size regions of interest in mice injected with bolus LVs or implanted with PEGDA–PLL matrices immobilized with LVs. f Representative radiance of 3 mice per group is shown. Each line represents one animal and each point reflects the radiance captured at a particular time. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. bolus LVs delivery, Student’s t-test. GFP green fluorescent protein PEGDA polyethylene glycol diacrylate, PLL poly-L-lysine, CMV cytomegalovirus, IRES internal ribosome entry sites, RFP red fluorescent protein, Luc lucierfase