Fig. 1

PEGDA–PLL presents a biocompatible porous 3D matrix. a Schematic of the preparation of bioactive PEGDA–PLL matrix. b Quantification of PLL retention on the matrix. c Scanning electron microscopy (SEM) images of PEGDA and PEGDA–PLL matrices showing macroporous structure throughout the scaffold. Scale bar = 100 µm. d Pore size of matrices measured using ImageJ with at least 100 pores from 3 representative images. e Viability of splenocytes cultured in these matrices measured at different times using MTT assay. f Retention of T-cells in these matrices after 24 h of culture measured using total viable cell count assay. ^***P < 0.001, one-way ANOVA with Tukey’s test or Student’s t-test. PEGDA polyethylene glycol diacrylate, PLL poly-L-lysine, 3D three dimensional, APS ammonium persulphate, TEMED N,N,N′,N′-tetramethylethylenediamine, MTT 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide, EDC 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide, NHS N-hydroxy-succinimide, TCP tissue culture plate