Fig. 2

ADPGK overexpression promotes PCa cell proliferation and migration in vitro. a ADPGK protein expression across prostate cell lines validated by Western blotting. b Western blotting results showed the stable overexpression of ADPGK in 22Rv1 and PC3 cells after lentivirus transfection. c Cell proliferation was measured by EdU assays (n = 3). Scale bar = 20 μm. d Cell viability was assessed by CCK-8 assays (n = 3). e Cell proliferation was measured by EdU flow cytometry analysis (n = 3). f Cell migration was assessed by Transwell assays (n = 3). Scale bar = 50 μm. g Cell migration was assessed by wound healing assays (n = 3). Scale bar = 200 μm. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ADPGK ADP-dependent glucokinase, PCa prostate cancer, SD standard deviation, OE overexpression, OD optical density, a.u. artificial unit