Fig. 4

Overexpression of Adipsin inhibits mitochondrial translocation of Irak2. a Western blotting results and quantitative analysis of Irak2 expression in cytoplasm and mitochondria of cardiomyocytes in the CHD + NTg, CHD + Adipsin-Tg, NTg + HFD, and Adipsin-Tg + HFD groups (n = 6). b Electron microscopy images of Irak2 stained with immunogold and quantitative analysis of Irak2-positive dots (red arrow) in the myocardial mitochondria of NTg or Adipsin-Tg mice fed with CHD or HFD (n = 5). Scale bar = 200 nm. c Western blotting results and quantitative analysis of Phb and Opa1 in myocardial mitochondria of NTg or Adipsin-Tg mice fed with CHD or HFD (n = 6). Statistical significance was determined using one-way ANOVA. All data are represented with mean ± SD. ^†P < 0.05 vs. CHD + NTg; ^‡P < 0.05 vs. CHD + Adipsin-Tg; ^¶P < 0.05 vs. HFD + NTg. Mito mitochondria, Cyto cytosol, CHD chow diet, HFD high-fat diet, Adipsin-Tg Adipsin tissue-specific transgenic mice, NTg nontransgenic mice, Irak2 interleukin-1 receptor-associated kinase-like 2, VDAC1 voltage-dependent anion channel 1, Phb prohibitin, Opa1 optic atrophy protein 1