Fig. 1

Adipsin is significantly downregulated in HFD-induced DCM. a Western blotting results and quantitative analysis of serum Adipsin levels in the treatment groups at 0, 2, 4, and 6 months (n = 4). b Western blotting results and quantitative analysis of Adipsin expression in WAT, interscapular BAT and heart tissue lysates from CHD- and HFD-fed mice (n = 6). c, d Immunohistochemical/immunofluorescence stainings and quantitative analysis of Adispin in mouse hearts from CHD- and HFD-fed groups (n = 5). Scale bar = 50 μm. Statistical significance was determined by two-tailed Student’s t test. All data are represented with mean ± SD. ^*P < 0.05 vs. CHD; CHD chow diet, HFD high-fat diet, WAT white adipose tissue, BAT brown adipose tissue, TF transferrin, IOD integrated option density, DAPI 4’,6-diamidino-2-phenylindole