Fig. 6

The CD68⁺CD163⁺ macrophage with high expression of Cav-1 accelerated iron homeostasis disorder in NAFLD. a Number of F4/80⁺CD11b⁺ macrophages in liver tissue were detected by flow cytometry (n = 3). b Expression of CD11b⁺ and F4/80⁺ in liver tissue were detected by immunohistochemical method between NC group and HFD group (n = 4). c CD68 and CD163 immunofluorescence co-staining results in liver tissue (n = 4). d CD163 and Ter-119 immunofluorescence co-staining results in liver tissue (n = 4). e Expressing of HO-1, CD68 and Fpn1 in liver tissue were detected by multiplex fluorescence immunohistochemical staining method (n = 3). f Multiplex fluorescence immunohistochemical staining method to detect the activation of F4/80⁺CD68⁺ macrophages and the expression of Cav-1 in liver tissue (n = 3). g Western blotting detected the expression of Cav-1, HO-1 and Fpn1 proteins in RAW264.7 cells with or without PA + OA (n ≥ 3). h Western blotting detected the expression of HO-1 in RAW264.7 cell between shRNA^Cav-1 treatment and shRNA^NC treatment (n = 3). i Western blotting detected the expression of HO-1 in RAW264.7 cell between shRNA^Cav-1 treatment and shRNA^NC treatment with or without PA + OA (n = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 as determined by Student’s t test analysis or one-way ANOVA. All data were shown as the mean ± SD. Cav-1 caveolin-1, NAFLD non-alcoholic fatty liver disease, HFD high-fat diet, HO-1 heme oxygenase-1, Fpn1 ferroportin 1