Fig. 5

Cav-1 deficiency in hepatocytes aggravated the accumulation of liver Fe²⁺ in NAFLD. a Schematic construction of hepatocellular-specific Cav-1 knockdown mice. b HE staining and Nile Red staining results of liver tissue. c The concentration of serum Cav-1 between Flox mice and Cav-1-CKO mice with or without high-fat diet (HFD) (n = 4). d The concentration of serum total iron, serum Tf, serum hepcidin, and serum ferritin in different groups (n = 4). e The concentration of Fe²⁺ and Fe³⁺ in liver tissue between Flox mice and Cav-1-CKO mice with or without HFD (n = 4). f Prussian blue staining results in different groups. g The expression of FTL in liver tissue was detected by Western blotting (n = 3). h Immunohistochemical method detected the expression of FTL and FTH protein in liver tissue (n = 4). i The expression of Fpn1 and hepcidin in liver tissue between Flox mice and Cav-1-CKO mice with HFD (n ≥ 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, as determined by Student’s t test analysis or one-way ANOVA. All data were shown as the mean ± SD. Cav-1-CKO Cav-1-knockout mice, Cav-1 caveolin-1, NAFLD non-alcoholic fatty liver disease, Tf transferrin, FTL ferritin light chain, FTH ferritin heavy chain, Fpn1 ferroportin 1, PA palmitic acid, OA oleic acid