Fig. 4

Cav-1 alleviated the development of NAFLD by up-regulating the expression of FTL/FTH. a Immunohistochemical staining results of Cav-1 in liver tissue between AAV9^Cav-1 and AAV9^NC mice with or without high-fat diet (HFD) (n = 4). b Concentration of serum TC between AAV9^NC and AAV9^Cav-1 with or without HFD (n = 3). c Oil Red O staining and Nile Red staining detected lipid accumulation in liver tissue. d Immunohistochemical staining detected the expression of SREBP1 protein in liver tissue (n = 4). e Concentration of serum total iron, liver tissue Fe²⁺ and liver tissue Fe³⁺ between AAV9^Cav-1 and AAV9^NC mice with or without HFD (n = 4). f Concentration of serum ferritin and serum Tf in different groups (n = 4). g Immunohistochemical staining was used to detect the expression of FTL and FTH in liver tissue (n = 3). h Transmission electron microscopy to detect the changes in the mitochondrial structure of hepatocytes (n = 3). Black arrows indicate mitochondrion. i Immunohistochemical method detected the expression of 4-HNE protein in liver tissue (n = 4). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, as determined by one-way ANOVA. All data were shown as the mean ± SD. AAV9 adeno-associated virus 9, TC total cholesterol, SREBP1 sterol regulatory element binding transcription factor 1, Tf transferrin, FTL ferritin light chain, FTH ferritin heavy chain, 4-HNE 4-hydroxynonenal