Fig. 3

Cav-1 promoted the storage of iron in the cells as Fe³⁺. a Western blotting detected the expression of Cav-1 between AAV9^NC and AAV9^Cav-1 (n = 3). b Immunofluorescence staining results showed the green fluorescence (EGFP) and red fluorescence (Cav-1) of liver tissue overlapped in the AAV9^Cav-1 group. The red fluorescence of hepatocytes in the AAV9^Cav-1 group was significantly increased (n = 3). c Concentration of serum total iron, serum ferritin and serum Tf between AAV9^Cav-1 and AAV9^NC mice with or without high-iron diet (n = 4). d Prussian blue staining results in different groups between AAV9^Cav-1 and AAV9^NC mice with high-iron diet. e The concentration of liver tissue Fe²⁺ between AAV9^Cav-1 and AAV9^NC mice with or without high-iron diet (n = 4). f Western blotting results of FTL in liver tissue between AAV9^Cav-1 and AAV9^NC mice with or without high-iron diet (n = 3). g Immunohistochemical staining was used to detect the expression of FTH in liver tissue between AAV9^Cav-1 and AAV9^NC mice with or without high-iron diet (n = 4). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, as determined by Student’s t test analysis or one-way ANOVA. All data were shown as the mean ± SD. AAV9 adeno-associated virus 9, Cav-1 caveolin-1, Tf transferrin, FTL ferritin light chain, FTH ferritin heavy chain