Fig. 8
From: Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges
An illustration shows the main strategy to overcome multiple sites targeting or one site targeting through the CRISPR/Cas system. The CRISPR/Cas system targets one miRNA, which in turn suppresses miRNA to bind numerous genes and limit the synthesis of tumor protein. The CRISPR/Cas system is designed to target numerous miRNAs, which in turn restrict the same oncogenic gene, thereby limiting the growth of tumors. The CRISPR/Cas system is intended to target a large number of miRNAs, which then block the activity of the same oncogenic gene. As a result, the progression of tumor cell is suppressed or slowed down. CRISPR clustered regularly interspaced short palindromic repeats, sgRNA single-guide RNA, Cas CRISPR-associated protein