Table 1 Primers for overexpression plasmids
From: Aspartoacylase suppresses prostate cancer progression by blocking LYN activation
Gene | Sequence 5′–3′ | |
|---|---|---|
ASPA | F | TCGGGTTTAAACGGATCCATGACTTCTTGTCACATTGCTG |
| Â | R | GGGCCCTCTAGACTCGAGCTAATGTAAACAGCAGCGAATAC |
C-Jun | F | TCGGGTTTAAACGGATCCATGACTGCAAAGATGGAAACGACC |
| Â | R | GGGCCCTCTAGACTCGAGTCAAAATGTTTGCAACTGCTGC |
LYN | F | TCGGGTTTAAACGGATCCATGGGATGTATAAAATCAAAAGGGAAAGACAGC |
| Â | R | GGGCCCTCTAGACTCGAGCTAAGGCTGCTGCTGGTATTGC |
SRPK1 | F | TCGGGTTTAAACGGATCCGCCACCATGGAGCGGAAAGTGCTTGC |
| Â | R | GGGCCCTCTAGACTCGAGGGAGTTAAGCCAAGGGTGCCG |
CSNK2A1 | F | TCGGGTTTAAACGGATCCGCCACCATGTCGGGACCCGTGCC |
| Â | R | GGGCCCTCTAGACTCGAGCTGCTGAGCGCCAGCGG |
FYN | F | TCGGGTTTAAACGGATCCATGGGCTGTGTGCAATGTAAG |
| Â | R | GGGCCCTCTAGACTCGAGTTACAGGTTTTCACCAGGTTGGTAC |
GAK | F | TCGGGTTTAAACGGATCCGCCACCATGTCGCTGCTGCAGTCG |
| Â | R | GGGCCCTCTAGACTCGAGGAAGAGGGGCCGGGAGC |
LYN (1-230) | F | TCGGGTTTAAACGGATCCATGGGATGTATAAAATCAAAAGGGAAAGACAGC |
| Â | R | GGGCCCTCTAGACTCGAGCTACTTGGGACTAATACAAGCCTTCTCC |
LYN (231-512) | F | TCGGGTTTAAACGGATCCCCACAGAAGCCATGGGATAAAGATGC |
| Â | R | GGGCCCTCTAGACTCGAGCTAAGGCTGCTGCTGGTATTGC |
ASPA (1-212) | F | TCGGGTTTAAACGGATCCATGACTTCTTGTCACATTGCTG |
| Â | R | GGGCCCTCTAGACTCGAGCTATCCTTCATTGAAATGATGTATAAAATCAAGAG |
ASPA (213-313) | F | TCGGGTTTAAACGGATCCAAAGAATTTCCTCCCTGCGC |
| Â | R | GGGCCCTCTAGACTCGAGCTAATGTAAACAGCAGCGAATAC |