Fig. 7
From: Aspartoacylase suppresses prostate cancer progression by blocking LYN activation
LYN mediates the function of ASPA in PCa cells. a Western blotting assay showed ASPA expression and the activity of LYN, JNK1/2, and C-Jun in PC-3 cells cotransfected with ASPA overexpression vector and/or LYN overexpression vector. b The cell proliferation ability of PC-3 cells cotransfected with ASPA overexpression vector and/or LYN overexpression vector was assessed using CCK-8 assay. c Western blotting assay showed ASPA expression and the activity of LYN, JNK1/2, and C-Jun in PC-3 cells transfected with shASPA and/or treated with the LYN inhibitor bafetinib. d The proliferation ability of PC-3 cells transfected with shASPA and/or treated with bafetinib was assessed using a CCK-8 assay. e Representative images of subcutaneous xenograft tumors derived from PC-3 cells transfected with shASPA and/or treated with bafetinib. f The volume of xenograft tumors derived from PC-3 cells transfected with shASPA and/or treated with bafetinib (n = 8). g The weight of xenograft tumors derived from PC-3 cells transfected with shASPA and/or treated with bafetinib (n = 8). h RT-qPCR results of proliferation-related genes and epithelial–mesenchymal transition genes in xenograft tumors derived from PC-3 cells transfected with shASPA and/or treated with bafetinib. The mRNA expression levels were normalized to ACTB levels. i Western blotting analysis (left) and quantification (right) of ASPA and the phosphorylation of LYN, JNK1/2, and C-Jun in subcutaneous xenograft tumors derived from PC-3 cells transfected with shASPA and/or treated with bafetinib. Protein expression levels were normalized to β-actin levels. The data are presented as the mean ± standard deviation (SD). ASPA aspartoacylase, CCND1 cyclin D1, CDH1 Cadherin 1, CCK-8 cell counting kit 8, C-Jun v-Jun avian sarcoma virus 17 oncogene homolog, DMSO dimethyl sulfoxide, JNK c-Jun N-terminal kinase, LYN Lck/Yes-related novel protein tyrosine kinase, OD optical density, RT-qPCR real-time quantitative PCR, PCNA proliferating cell nuclear antigen, shRNA small hairpin RNA. *P < 0.05, **P < 0.01, ns not significant