Fig. 1From: Recent developments in application of single-cell RNA sequencing in the tumour immune microenvironment and cancer therapyThe main procedure for single-cell RNA sequencing. The main procedure for single-cell RNA sequencing includes separation and extraction of single cells (a), reverse transcription amplification, generation of cDNA libraries, sequencing (b) and Data analysis (c). FASC fluorescence-activated cell sorting, UMI unique molecular identifier, cDNA complementary DNABack to article page